Fig. 1
From: Characterization of a chemically induced osteoarthritis model in zebrafish

The zebrafish jaw joint was designated as a modelling joint. (A) Col2a1a: EGFP expression was widely observed on the surface cartilage of adult zebrafish jaw joint. (B–D) Alizarin red-Alcian blue staining of the whole skull of adult zebrafish and two states of the jaw joint were presented, including closed and opened. (E) Schematic diagram of zebrafish immobilization, in which zebrafish were immobilized in a supine position in an anesthetic-containing water, with the anterior end of the jaw placed in a groove at the top, and the jaw joint opened.