Fig. 2
From: Amyloids in bladder cancer hijack cancer-related proteins and are positive correlated to tumor stage
Electrophoretic and size exclusion chromatographic analyses of cell line amyloids. (a) ThT staining of a 1.5% agarose gel native electrophoresis of protein homogenates treated with proteinase K (+ PK) or not treated (-PK). Protein homogenates from blood cells, RT4, EJ138, and HT1197 cell lines were included. The loaded sample in each well is indicated above each lane. Asterisks indicate the presence of positive ThT bands, which appear more evident in the signal on the weaker fluorescent background smear. (b) The same gel as in a, stained with Coomassie Blue. (c) Chromatogram obtained during gel filtration of HT1197 protein homogenate mixed with ThT, using Superdex matrix 200 (range: 3-600 kDa), recorded with in-line PDA (absorbance at 280 nm, 10− 3 AU, blue line) and FD (Ex350 nm and Em472 nm, mV, black line). X-axis is elution volume (mL). Arrows indicate void volume (Vo) and total volume (Vt). Asterisks indicate ThT-positive peaks.
