Fig. 6
From: Amyloids in bladder cancer hijack cancer-related proteins and are positive correlated to tumor stage
Detection of amyloids in mouse bladder tumors. (a) Normalized ThT fluorescence intensity measured by spectrofluorimetry of bladder homogenates. Control (C) and BBN-treated mice at 16 or 23 weeks are compared. p-value between groups is indicated. (b) Correlation graph between normalized ThT fluorescence intensity and observed histological changes. ThT fluorescence intensity was measured by spectrofluorimetry of bladder homogenates from control (C) or BBN-treated mice at 16 or 23 weeks. LPIC: lamina propria invasive carcinoma; MPIC: muscularis propria invasive carcinoma. ANOVA linear correlation coefficient and p-value are shown. (c–f) Focal planes of bladder sections stained with ThT (green) and TO-PRO-3 iodide (red) at 10x magnification. Histopathological diagnosis is indicated. bl: bladder lumen; u: urothelium; lp: lamina propria; mp: muscularis propria. Bar: 100 μm. (g–j) Focal planes of bladder section stained with ThT (green) and TO-PRO-3 iodide (red) at 63x magnification. Histopathological diagnosis is indicated. c: cytoplasm; n: nucleus; e: extracellular. Bar: 10 μm.
