Fig. 4 | Scientific Reports

Fig. 4

From: Electrophysiologically calibrated optogenetic stimulation of dentate granule cells mitigates dendritic spine loss in denervated organotypic entorhino-hippocampal slice cultures

Fig. 4

Physiological properties of ChR2-expressing GCs are indistinguishable from non-transduced controls. (a) Whole-cell patch-clamp recordings of dentate GCs were performed in current-clamp mode. Current steps ranging from − 100 pA to 300 pA, in 10 pA increments, were injected. Passive and active properties of ChR2-tdTomato positive and negative GCs from virus-injected cultures were compared. (b) The frequency of APs in response to current injection was similar between ChR2-positive (n = 16) and ChR2-negative GCs (n = 9). (c, d) There were no significant differences in the first AP threshold (Brunner-Munzel test, P = 0.82), nor the rheobase (P = 0.20) between the two cohorts. (e-h) We observed no significant differences in the resting membrane potential (P = 0.46), the input resistance (P = 0.17), the membrane time constant (P = 0.23), nor the membrane capacitance (P = 0.62) between ChR2-positive and ChR2-negative GCs. n represents number of cells. Bars represent means ± SEM.

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