Fig. 5
From: Dissecting the sterility phenotype in gene edited Drosophila suzukii pgSIT males
pgSIT sterile males cannot transfer sperm in wildtype matings. Reproductive tracts dissected from mated females were used to assess DNA transfer from males. Using a primer set that amplifies a 731 bp segment of the vasCas9 transgene, PCRs were performed to detect transfer of sperm from wildtype females mated to vasCas9 males. The vasCas9 amplicon was detected in the reproductive tracts of females mated to vasCas9 males. The positive and negative controls behaved as expected. No vasCas9 amplicon was detected in reproductive tracts of females mated with pgSIT sterile males and gRNAsxl, βtub males, nor in virgin females. A 1 kB ladder flanks the ends of the gel.
