Fig. 8 | Scientific Reports

Fig. 8

From: Ankrd1 as a potential biomarker for the transition from acute kidney injury to chronic kidney disease

Fig. 8

Validated of hub genes in vivo experments. The RT-qPCR analysis of Ankrd1 (A) and Sema5a (B) in the progression of AKI-CKD. Images of Ankrd1 immunohistochemistry staining from mouse kidney sections (D) and the percentage of the positive area (C). Scale bar: 50 μm; magnification: 200×; (C-D) Histological and immunohistochemical analysis for proximal tubule cells marker LTL (E) and glomeruli cells marker WT1 (F) in uIRI kidney tissue. Scale bar: 20 μm; magnification: 400×. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. Sham group; n = 4. IRI, ischemia-reperfusion injury; LTL, Lotus Tetragonolobus Lectin.

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