Table 1 Sequences for RT-qPCR primers.
Gene | Forward primer (5’ to 3’) | Reverse primer (3’ to 5’) | References |
|---|---|---|---|
FABP5 | 5’-GGACAGCAAAGGCTTTGATG-3’ | 5’-GCTCATTGAACTGAGCTTGG-3’ | 30 |
CD36 | 5’-ATGTAACCCAGGACGCTGAG-3’ | 5’-GTCGCAGTGACTTTCCCAAT-3’ | 30 |
PPARγ | 5’-AAGGCCATTTTCTCAAACGA-3’ | 5’-GATGCAGGCTCCACTTTGAT-3’ | 30 |
SREBP | 5’-ACAGTGACTTCCCTGGCCTAT-3’ | 5’-GCATGGACGGGTACATCTTCAA-3’ | 31 |
FASN | 5’-AAGGACCTGTCTAGGTTTGATGC-3’ | 5’-TGGCTTCATAGGTGACTTCCA-3’ | 31 |
SCD1 | 5’-TCTAGCTCCTATACCACCACCA-3’ | 5’-TCGTCTCCAACTTATCTCCTCC-3’ | 31 |
LDLR | 5’-ACCAACGAATGCTTGGACAAC-3’ | 5’-ACAGGCACTCGTAGCCGAT-3’ | 31 |
ACACA | 5’-ATGTCTGGCTTGCACCTAGTA-3’ | 5’-CCCCAAAGCGAGTAACAAATTCT-3’ | 31 |
GRP78 | 5’-CATCACGCCGTCCTATGTCG-3’ | 5’-CGTCAAAGACCGTGTTCTCG-3’ | 32 |
ATF4 | 5’-CGAGGTGTTGGTGGGGGACTTGA-3’ | 5’-CAACCCATCCACAGCCAGCCATT-3’ | 32 |
XBP1 | 5’-AACCTGTAGAAGATGACCTCGTTCC-3’ | 5’-AAAGAGTTCATTGGCAAAAGTTCCAG-3’ | 32 |
CHOP | 5’-CCCTCACTCTCCAGATTCCAGTC-3’ | 5’-CTAGCTGTGCCACTTTCCTTTCA-3’ | 32 |
GAPDH | 5’-TCAAGAAGGTGGTGAAGCAGG-3’ | 5’-TCAAAGGTGGAGGAGTGGGT-3’ | 32 |
ATF6 | 5’-CTGATGGCTGTTCAATACACAG-3’ | 5’-GATCCCTTCGAAATGACACAAC-3’ | 33 |
PERK | 5’-CCAGTTTTGTACTCCAATTGCA-3’ | 5’-CAGATACAGCTGGCCTCTATAC-3’ | 33 |
