Fig. 2

Experimental process in the ANL. (A) Workflow to measure the cell growth (shown in yellow-green) and of the product amount (shown in orange) of glutamic acid-producing cells. The cell growth was evaluated by the optical densities from the cells at 600 nm. The product amount was evaluated by the colorimetric determination: The diluted supernatants were reacted with reagents I and II of the glutamic acid-quantification kit, and then the absorption of the mixtures was monitored at 555 nm after incubation at room temperature for 20 min. (B) Workspace on the liquid handler. The transfer robot transfers the plates from some devices to the liquid handler. The pipette dispenses samples in the well plate and solvents and reagents in the reservoir into each well of the other empty well plate for dilution or assay.