Fig. 1

Seed counting in Rf975 and HA89 capitula at different developmental stages (R6 and R8) and flow cytometry analysis of R6 developing embryos. (A–D) Photographs of capitula (A,C) and image analysis outputs (B,D) from the Rf975 line at R6 (A,B) and R8 (C,D) developmental stages. The corolla, anthers, and style/stigma were removed. (E–H): Photographs of capitula (E,G) and image analysis outputs (F,H) from the HA89 line at R6 (E,F) and R8 (G,H) developmental stages. (I,J): Flow cytometry analysis of ovaries from Rf975 (I) and HA89 (J) at R6. Ploidy level, median PI-A (propidium iodide fluorescence) value, and coefficient of variation (CV) are shown alongside the graphs. Scale bars = 2 cm.