Fig. 3
Progeny test principal component analysis and cytoembryological analysis of Rf975 × HA89 hybrids. (A) Canonical single sexual embryo sac. The central cell (cc), fused polar nuclei (fpn), and egg apparatus (ea) are visualized at the micropylar region (m); antipodals (a) are visible at the distal end. The three degenerating megaspore remnants appear to be crushed against the micropyle. (B–D) Aposporous embryo sac (AES) with fused polar nuclei (fpn), adjacent to the canonical sexual embryo sac (SES) with antipodal cells (a), fused polar nuclei (fpn), and egg apparatus (ea). Bars: 100 μm. (E) Principal component analysis of molecular markers obtained from the MCSeED-based experiment, representing the genetic/epigenetic distances among the progenitors (Rf975: experimental codes apo27-28-29; HA89: experimental codes sex 24-25-26) and F1, F1-E, F2-2x, and F2-3x plants.
