Fig. 3
From: Inhibition of TGF-β signaling enhances osteogenic potential of iPSC-derived MSCs
Gene expression of MSC- and iPSC-markers during iMSC-differentiation. Expression of (A) MSC-markers (CD44, CD73) and (B) of iPSC-markers (OCT4, NANOG, TERT) was measured by qPCR. Absolute mRNA copy numbers of iPSCs (red), JPCs (orange) and iMSCs differentiated with (green) and without SB (blue) at different passages of the differentiation process were normalized to the housekeeping gene glycerinaldehyde-3-phosphate dehydrogenase (GAPDH) and x-fold induction values relative to the iPSC sample were calculated. Means + SD (n = 4) are displayed and were compared by one-way ANOVA and Tuckey’s multiple comparison test (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; # indicate significant p-values compared to iPSCs).
