Fig. 5
From: Inhibition of TGF-β signaling enhances osteogenic potential of iPSC-derived MSCs
Osteogenic potential of differently generated iMSCs. iMSCs were differentiated using four different protocols (SB_d10 = SB incubation in P0 until d10, SB_d5 = SB incubation in P0 until d5, SB_P1 = SB incubation in P1 for 10 days, -SB = without SB). Differences in mineralization of iMSCs of passages 2–4 was visualized by alizarin red staining after 21 days of osteogenic differentiation. (A) Increase in mineralization of differently generated iMSCs of different passages after stimulation with osteogenic medium for 21 days. Percentage of mineralizing samples was determined by counting mineralized and non-mineralized wells after alizarin staining. (B) Mean alizarin concentrations and SEM are displayed as connected lines to visualize development of osteogenic potential in different passages. (C) Alizarin concentrations of osteogenically differentiated iMSCs were quantified photometrically and means + SD are displayed and compared by 2-way ANOVA and Tuckey’s multiple comparison test. (D) Alizarin concentration of iMSCs of passage 4 and originating JPCs treated with osteogenic (OB) and control medium (CO) was quantified photometrically and means + SD are displayed and compared by 2-way ANOVA and Tuckey’s multiple comparison test (n = 8, * p < 0.05, ** p < 0.01). (E) Representative images of differently generated iMSCs after osteogenic differentiation in passage 4 and alizarin staining (4x magnification, scale bar = 500 μm).
