Fig. 6
From: Inhibition of TGF-β signaling enhances osteogenic potential of iPSC-derived MSCs
Senescence-associated β-galactosidase (SA-β gal) activity and expression of senescence marker genes during iMSC-differentiation. (A) Gene expression of P16 and P21 of iPSCs (red), JPCs (orange) and iMSCs differentiated with (green) and without SB (blue) at different passages of the differentiation process was quantified by qPCR. Absolute mRNA copy numbers were normalized to the housekeeping gene glycerinaldehyde-3-phosphate dehydrogenase (GAPDH) and x-fold induction values were calculated relative to the iPSC’s values. Means + SD (n ≥ 3) are displayed. (B) Activity of SA-β gal was measured by flow cytometry. Means of median fluorescence index (MFI) values + SD (n ≥ 3) are displayed. Gene expression and SA-β gal activity data was analyzed using one-way ANOVA and Tuckey’s multiple comparison test (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, # indicate significant p-values compared to iPSCs).
