Fig. 5
From: Aberrant activation of the mTOR signaling pathway in Rasmussen encephalitis
Dysmorphic neurons and microglial nodules in the cortex and white matter of RE exhibit strong positive expression of Beclin-1, while astrocytes show negative expression. A-F. Representative tissue images of Beclin-1 in the control, RE, FCD. The expression of Beclin-1 was observed in the cortical neurons of the control and FCD groups (A-B, E-F), while its expression was less prominent in the white matter. (C-D) demonstrates a high expression of Beclin-1 in the dysmorphic neurons in the cortex and ectopic neurons in the white matter of RE. Neuronophagia are observed in the white matter, with phagocytic cells arranged in a ring-like pattern around neuronal cell bodies or distributed in a vertical arrangement along the axonal regions of the neurons. G-O. Representative fluorescent staining images of Beclin-1 in RE tissue sections. Beclin-1 demonstrates strong co-localization with microglial nodules (G-I) and dysmorphic neurons (J-L), but no co-labeling was observed with astrocytes (M-O). The nuclear stained with DAPI appear blue. Scale bars represent 50 μm.
