Table 2 Kinetic parameters of WT-AFUOX and Q269L mutant.

From: The role of Gln269Leu mutation on the thermostability and structure of uricase from Aspergillus flavus

 

Km (mM)

Specific activity (U/mg)

Kcat=TON (sec−1)

Kcat/Km

(mM−1. S−1)

WT-AFUOX

0.089 ± 0.034

4.018 ± 0.243

3.125 ± 0.061

35.112 ± 2.80

Q269L

0.070 ± 0.016

3.926 ± 0.129

3.725 ± 0.087

53.217 ± 4.19

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