Table 1 The antibodies employed for staining and flow cytometric analysis of cell phenotypes.

From: Comparative analysis and process optimization for manufacturing CAR-T using the PiggyBac system derived from cryopreserved versus fresh PBMCs

Antibody name

Manufacturer

Catalog Number

Alexa Fluor® 488 anti-human CD3

BioLegend Inc., San Diego, California

300,415

PE anti-human CD16

Thermo Fisher Scientific, Waltham, Massachusetts

12–0168-42

PerCP/Cyanine5 anti-human CD45

BioLegend Inc., San Diego, California

304,028

APC anti-human CD19

BioLegend Inc., San Diego, California

302,212

Brilliant Violet 421 anti-human CD56

BioLegend Inc., San Diego, California

318,318

Alexa Fluor 700 anti-human CD3

BioLegend Inc., San Diego, California

557,943

Alexa Fluor®488 anti-human CD4

BioLegend Inc., San Diego, California

344,618

PerCP-Cyanine5. 5 anti-human CD8a

Thermo Fisher Scientific, Waltham, Massachusetts

45–0088-42

Brilliant Violet 605 anti-human CD45RO

BioLegend Inc., San Diego, California

304,238

PE/Cyanine7 anti-human CCR7

BioLegend Inc., San Diego, California

353,226

Biotin Human Mesothelin protein

Genscript Biotech, Nanjing, China

N/A

PE Streptavidin

BD Biosciences, San Jose, California

554,061

PE/Cyanine7 anti-human Lag-3

BioLegend Inc., San Diego, California

369,310

Brilliant Violet 650 anti-human Tim-3

BD Biosciences, San Jose, California

565,564

BV786 Mouse Anti-Human CD27(L128)

BD Biosciences, San Jose, California

563,327