Fig. 5

Flow cytometric analysis of changes in mitochondrial membrane potential (MMP) referred as Δψm are expressed as mean fluorescence intensity/cell (MFI) using rhodamine 123 as fluorescent dye. MMP was detected in intact splenocytes, control cells (Ctrl) and cells treated with 5 or 50 μmol/ml of silybin (SB), silychristin (SCH), and 2,3-dehydrosilybin (DHSB) after 24 h incubation (a, b). (c) Representative histograms for 50 μmol/ml. Values represent means ± SD (n = 6). Significantly different values between groups indicated by lines are marked as: *p < 0.05, **p < 0.01, ***p < 0.001, ns—not significant.