Fig. 7 | Scientific Reports

Fig. 7

From: Flavonolignans silybin, silychristin and 2,3-dehydrosilybin showed differential cytoprotective, antioxidant and anti-apoptotic effects on splenocytes from Balb/c mice

Fig. 7

Relative gene expression of antioxidant genes (a–d) Nrf2, SOD1, CAT and GPx analysed by real-time PCR in control cells (Ctrl) and cells treated with 5 or 50 μmol/ml of silybin (SB), silychristin (SCH), and 2,3-dehydrosilybin (DHSB) after 24 h incubation. Data (Ct values, n = 6) were normalised to housekeeping gene (GAPDH) and relative gene expression was calculated using the 2−∆∆Ct method using intact cell data as a calibration value. Significantly different values between groups indicated by lines are marked as: ***p < 0.001, ns—not significant.

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