Fig. 1 | Scientific Reports

Fig. 1

From: A robust strategy for overexpression of DNA polymerase from Thermus aquaticus using an IPTG-independent autoinduction system in a benchtop bioreactor

Fig. 1The alternative text for this image may have been generated using AI.Fig. 1The alternative text for this image may have been generated using AI.

Analysis of primer specificity, efficiency, and absolute quantification. (a) Qualitative assessment of primer specificity for DH5α, pBR322, and pD451-SR_TaqPol at annealing temperatures of 55, 60, and 65 °C using gel electrophoresis. Quantitative assessment of primer specificity using qPCR for (b) DH5α, (c) pBR322, and (d) pD451-SR_TaqPol, respectively. Evaluation of primer efficiency at various DNA template concentrations for (e) DH5α, (f) pBR322, and (g) pD451-SR_TaqPol, respectively.

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