Fig. 6 | Scientific Reports

Fig. 6

From: A robust strategy for overexpression of DNA polymerase from Thermus aquaticus using an IPTG-independent autoinduction system in a benchtop bioreactor

Fig. 6The alternative text for this image may have been generated using AI.

Assessment of pure Taq-pol using SDS-PAGE and western blot. (a) SDS-PAGE analysis of purified Taq-pol from small-scale culture. Lane M: protein ladder (Pierce™ Unstained Protein ladder, Thermo Fisher Scientific (USA)); lane 1: crude Taq-pol; lane 2: purified Taq-pol. The solid triangle indicates the target protein at 63 kDa. (b) Western blot analysis. Lane 1: purified Taq-pol; lane M: Protein ladder (Spectra Multicolor Broad Range Protein Ladder, Thermo Fisher Scientific (USA)).

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