Table 1 Primer sequences.

From: A robust strategy for overexpression of DNA polymerase from Thermus aquaticus using an IPTG-independent autoinduction system in a benchtop bioreactor

Primer

Primer types

Sequences (5′–3′)

Tm (°C)

Amplikon size (bp)

rrs_16SrRNA

Forward

GCAGGCGGTTTGTTAAGTCA

59.05

114

Reverse

TCACCGCTACACCTGGAATT

59.02

ori_pBR322

Forward

AACCGCGTAAGACACGACTT

58.96

105

Reverse

AGGCCACCACTTCAAGAACT

59.15

ori_pD451

Forward

AGTGTAGCCGTAGTTAGGCC

58.89

120

Reverse

AACCCGGTAAGACACGACTT

58.96

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