Fig. 5 | Scientific Reports

Fig. 5

From: Pollen–pistil interactions in divergent wide crosses lead to spatial and temporal pre-fertilization reproductive barrier in flax (Linum usitatissimum L.)

Fig. 5

Pollen germination in crosses with L. grandiflorum (a): Callose formation on the stigma surface (2 HAP) in Lu X Lg and, (b): Callose formation (2 HAP) Lg X Lu as indicated by the arrow mark pointing towards the aniline blue stained localized thickened callose deposition plugs visible under microscope; (c): Ungerminated pollen grains seen during longer hours after pollination in Lu X Lg (10 HAP) as indicated by the arrow mark pointing towards aniline blue stained small granular fluorescent areas; (d, e): Ungerminated pollen grains seen during longer hours after pollination in Lg X Lu (24 HAP) that start degradation/deformation; (f, g): Limited/restricted pollen grain germination on stigmatic surfaces in Lg X Lu (4 HAP) and Lu X Lg (6 HAP) wide crosses respectively.

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