Fig. 1

Flow diagram for the Clonal Neoantigen pipeline. The steps/ modules are numbered and described further in the main text. Output data from a step that is used as input for a downstream step is represented by blue arrows between steps. Red dotted line arrows indicate BAM files from the non-UMI alignment module flowing to the subsequent steps. Green arrows represent additional data fields from the output of variant calling and annotation and allelic copy number determination included in the PyClone variant level report.