Table 4 Coverage statistics for exome sequencing experiment.
Patient / Sample ID | Normal | Tumor | Vigil |
|---|---|---|---|
Total read pairs (millions) | 64.67 | 558.13 | 544.29 |
Aligned read pairs (millions) | 63.71 | 548.35 | 533.76 |
On target bases (millions, before UMI) | 6,233 | 53,178 | 51,925 |
On target bases (millions, after UMI) | 4,480 | 34,039 | 34,038 |
Percentage duplicate reads | 28% | 36% | 34% |
Mean target coverage before UMI processing | 171 | 1,459 | 1,424 |
Mean target coverage after UMI processing | 123 | 934 | 934 |
Percentage loss in coverage | 28.1% | 36.2% | 34.4% |
% Target bases at 50X (pre-UMI) | 98.4% | 98.8% | 98.8% |
% Target bases at 100X (pre-UMI) | 93.6% | 98.7% | 98.7% |
% Target bases at 250X (pre-UMI) | 6.8% | 98.5% | 98.5% |
% Target bases at 500X (pre-UMI) | 0.1% | 97.3% | 97.1% |
% Target bases at 50X (post-UMI) | 98.0% | 98.7% | 98.7% |
% Target bases at 100X (post-UMI) | 74.9% | 98.6% | 98.6% |
% Target bases at 250X (post-UMI) | 0.5% | 97.9% | 98.2% |
% Target bases at 500X (post-UMI) | 0.0% | 88.6% | 90.5% |
