Fig. 2
From: Crosado embalming related alterations in the morpho-mechanics of collagen rich tissues

Schematic representation of the process to obtain measurement of the cross-sectional areas. (a) Tissue cut into dumbbell shape using template. (b) Tissue placed on mount for clamping (yellow rectangle). (c) Custom clamps (blue square) placed around tissue at fixed points. (d) Polysiloxane impression material (purple shape) placed around central portion of the tissue. (e) Moulding clamps (white rectangle) placed around polysiloxane impression material to ensure good coupling between tissue and impression material, and left to harden. (f) Hardened impression material is removed (the sample is ready for testing). (g) Using a scalpel, polysiloxane impression material sectioned in half to visualised cross-section created by tissue. (h) Sectioned impressions scanned on flatbed scanner. (i) The cross section was traced on computer software to quantify the cross-sectional area.