Fig. 3
From: Development of a flexible 3D printed TPU-PVC microfluidic devices for organ-on-a-chip applications
Evaluation of myoblast differentiation, maturation, and alignment in the 3D-flexTPU-MFD. (a) Representative images of myoblasts cultured in the 3D-flexTPU-MFD on day 3 (D3), day 6 (D6), and day 9 (D9), stained with Calcein AM (green) and Hoechst 33342 (blue) to assess cell viability and proliferation. Scale bar: 200 μm. (b) Quantitative analysis of fluorescence intensity (left) and cell area (right) from myotube structures on D3, D6, and D9. MFI denotes mean fluorescent intensity. (n = 3–5; data are presented as mean ± SEM). p values were determined by Tukey HSD and Dunnett’s test. (c) Immunofluorescence staining of myosin heavy chain (MYH7) in a 96-well plate on D4, and in the 3D-flexTPU-MFD on D4 and D8. (d) Quantitative analysis presented in bar plot for comparing myotube fusion index (left) and violin plots for comparing myotube widths (right) in the 3D-flexTPU-MFD and in the 96-well plate (n = 3–5; data are presented as mean ± SEM). p values were determined by Tukey HSD. (e) F-actin staining of myoblasts cultured in the 3D-flexTPU-MFD and in a 96-well plate on D6. Scale bar: 200 μm. (f) Polar histograms representing the angular distribution of myotubes cultured in the 3D-flexTPU-MFD (left) and the 96-well plate (right) on D6.
