Fig. 3

PcAS-infected IFN-γ KO mice develop hyperparasitemia and increased kidney pathology. IFN-γ KO and WT C57BL/6 mice were infected with PcAS. (a) Peripheral parasitemia was determined on blood smears. (b) The clinical score and (c) body weight was monitored daily from day 6 p.i. onwards. Infected mice were euthanized and dissected at peak parasitemia at day 9 or 10 p.i. and (d) albumin/creatinine ratios were determined in urine samples. Asterisks above data points indicate significant differences compared to control mice, asterisks above a horizontal line show significant differences between infected groups. (e) Albumin/creatinine ratios were subdivided according to gender. Asterisks above data points indicate significant differences compared to IFN-γ WT infected mice of corresponding gender, asterisks above a horizontal line show significant differences between gender, *p < 0.05, **p < 0.01, ***p < 0.001. (f) Correlation analysis of parasitemia with albumin/creatinine ratios of urine of PcAS-infected IFN-γ WT and IFN-γ KO at day 7, 8, 9 and 10 p.i. Dark brown upside down triangles indicate PcAS-infected IFN-γ KO which produced dark urine. Spearman correlation test was performed, Spearman correlation coefficient r and p-values are shown. Data of two experiments, Control IFN-γ WT: n = 4, Control IFN-γ KO: n = 8, PcAS-infected IFN-γ WT mice: n = 13, PcAS-infected IFN-γ KO mice: n = 19. Mann-Whitney U test with Holm-Bonferroni correction for multiple testing (number of tests = 4) was performed in panels c-e.