Fig. 5
Hyaline casts are formed in PcAS-infected IFN-γ KO mice. At day 9 or day 10 p.i. (peak parasitemia) IFN-γ KO C57BL/6 mice infected with PcAS were dissected. (a) PAS staining and (b) H&E staining were performed (original magnification x40, bar = 50 μm). Casts are indicated with a black arrow on both the PAS and H&E staining. (c) Casts on H&E stained sections were counted. The gender of the infected IFN-γ KO mice is indicated by dark red for males and bright red for females. Asterisks above data points indicate significant differences compared to control mice, asterisks above a horizontal line show significant differences between KO and WT infected groups, *p < 0.05, **p < 0.01, ***p < 0.001. Data of two experiments, Control IFN-γ WT: n = 4, Control IFN-γ KO: n = 8, PcAS-infected IFN-γ WT mice: n = 13, PcAS-infected IFN-γ KO mice: n = 19. Mann-Whitney U test with Holm-Bonferroni correction for multiple testing (number of tests = 4) was performed in panel c.
