Fig. 3
From: MiR-340-5p regulates PD-L1 and predicts pembrolizumab response in extranodal NK/T-cell lymphoma
A negative correlation between miR-340-5p and miR-424-5p and PD-L1 expression in ENKTL samples. (A) Representative images of immunohistochemical stains showing patterns of PD-L1 expression in three patients with CPS score of 100 (Pt1, high), 30 (Pt2, intermediate), and 0 (Pt3, low). (B) The expression levels of miR-340-5p and miR-424-5p were lower in tumor tissue of patients (Pt1, Pt2) with high and intermediate PD-L1 expression than a patient with low PD-L1 expression (Pt3). The U6 served as the corresponding internal reference for cellular miRNA. (C) The expression levels of miR-340-5p and miR-424-5p in the serum of the same patient were confirmed by RT-qPCR. The synthetic spike control cel-miR-39 was used as an invariant control for the serum miRNA. (D, E) A negative correlation between miR-340-5p or miR-424-5p and PD-L1 CPS in the ENKTL tissues.
