Fig. 3 | Scientific Reports

Fig. 3

From: Antimicrobial cyclic peptides effectively inhibit multiple forms of Borrelia and cross the blood-brain barrier model

Fig. 3

Production of Cx7C peptides fused with O-BBB and assessment of their binding to Borrelia. (A) Amplicons encompassing Cx7C. (B) Purified Cx7C peptides fused with O-BBB (designated as Bor-1 to Bor-29) separated on SDS-PAGE and stained with Coomassie blue. (C) Molecular mass of the purified peptides measured by MALDI-MS. Full spectra are available in supplementary information 4. (D) Binding of the selected peptides on Borrelia assessed by ELISA. In assay wells were coated either Borrelia (Bb) or coating buffer (-), incubated with each peptide (Bor-1 to Bor-29) or phosphate buffer saline (PBS, negative control) or with O-BBB only (negative control). Error bars represent the standard deviation of triplicate measurements.

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