Fig. 6

Effect of Bor peptides on the eukaryotic cell metabolism, hemocompatibility and proliferation of eukaryotic cells. (A) Cell toxicity - effect of peptides on the cell metabolism tested by XTT. In control wells, the peptides were replaced either by PBS or Triton X (0.1%). Error bars represent the standard deviation of triplicate measurements. (B–E) Cell toxicity – effect of peptides on the proliferation of cells was assessed up to 120 h.
3.33 µM,
1.67 µM or
0 µM (only PBS) of peptides were used in assay. The Pearson correlation test coefficient (PCC) revealed no significant difference in cell proliferation in presence of PBS and peptides (r ranged from 0.98 to 0.99 for all peptides; P value < 0.005). (F) Hemocompatibility of the peptides tested for three different concentrations at three timepoints. Sheep erythrocytes were used to test any hemolysis caused by peptides. Erythrocytes incubated only with Triton X served as positive control.