Fig. 2

Characterizations of hucMSC-NExos and hucMSC-HExos. (A) TEM image of NExos and HExos (Scale bar = 100 nm). (B) Detection of the protein levels of hucMSC-Exos markers in NExos and HExos (negative for Calnexin and positive for CD81 and Syntenin) via WB analysis. (C–E) Evaluation of size and concentration of NExos and HExos via NTA. (F) The mRNA level of HIF1α in NExos and HExos was detected by qPCR. Data was statistically examined via Student’s t-test and illustrated as mean ± SEM (**p < 0.01).