Fig. 1
From: NlpD as a crucial factor in desiccation resistance and biofilm formation in Cronobacter sakazakii
Construction and growth characteristics of the nlpD mutant in C. sakazakii. (A) Schematic representation of the genomic location of nlpD (ESA_00549). The stop codon of nlpD is located 50 bases upstream of the start codon of the adjacent downstream gene ESA_00550. (B) Construction of the pCVD442 suicide plasmid for nlpD deletion in C. sakazakii. (C) Growth of WT, ΔnlpD and cp.nlpD in LB medium. WT: C. sakazakii ATCC BAA-894; ΔnlpD: nlpD knockout mutant strain; cp.nlpD: complementation strain. Each data point represents the average and standard deviation of three biological replicates.
