Table 1 Bacterial strains, plasmids and primers used in this study.
From: NlpD as a crucial factor in desiccation resistance and biofilm formation in Cronobacter sakazakii
Strains, plasmids, primers | Description | Reference |
|---|---|---|
Strains | ||
 WT | Wild-type Cronobacter sakazakii BAA-894 | ATCC |
 ΔnlpD | Markerless deletion mutant ΔnlpD | This study |
 ΔnlpD-C | nlpD complementation in ΔnlpD | This study |
 E. coli DH5α | Strain for construction | |
 S17 lambda pir | Strain for construction harbouring lambda pir | |
 S17 lambda pir-ΔnlpD | S17 lambda pir harbouring pCVD442-ΔnlpD | This study |
Plasmids | ||
 pACYC184 | Low-copy plasmid | |
 pACYC184-nlpD | nlpD complementation vector | This study |
 pCVD442 | Suicide plasmid for markerless deletion | |
 pCVD442-ΔnlpD | nlpD deletion plasmid | This study |
Primers (5′-3′) | ||
 pCVD442-fwd | GGCTGTCAGACCAAGTTTACTCATATATACTTTAGATTG |  |
 pCVD442-rev | GCAGATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTG |  |
 ΔnlpD-A | GAAAAAGGAAGAGTATCTGCGTTCTGAATCAACTGCGTGCTCAGG |  |
 ΔnlpD-B | GCACTCTGCCAATTTATCGCTGCGATGGCGGCATAATCATAATCATCC |  |
 ΔnlpD-C | CGATAAATTGGCAGAGTGCGCTTCTTCAG |  |
 ΔnlpD-D | GATTAATTGTCAAGGCTAGCGCAGACCGAAACGACGGGCCAGAAC |  |
 nlpD-comp-fwd | GGTCTAGATAGAGAAGTAAACCATTCCAG |  |
 nlpD-comp-fwd | GGTCTAGATCTTCATTTAAGTCATGAAC |  |
 ΔnlpD-E | GTGGAGTGAACGGGCAATGGTAAAC |  |
 ΔnlpD-F | GTGGACATCTTCAAGCGTTGCC |  |
