Fig. 4
LSD1 demethylates p53 in response to senescent stress. (a) U2OS cells transfected with siRNA for LSD1 and treated with 2 µM etoposide for 5 days were subjected to immunoblot analysis. (b) U2OS cells treated with 100 nM ORY-1001 and 2 µM etoposide for 5 days were subjected to immunoblot analysis. (c) U2OS cells transfected with p3XFLAG-LSD1 and treated with 2 µM etoposide for 5 days were subjected to immunoblot analysis. Protein levels relative to p53 levels were quantified using NIH ImageJ software. Original blots are presented in Supplementary Information. Data are representative of two independent experiments and values are shown as mean ± s.e.m.
