Fig. 6
MiR-10a-5p overexpression suppressed inflammatory response in SCI rats and reduced neuropathic pain. LV-miR-10a-5p was injected into the spinal cord of SCI model rats. (A). QRT-PCR was used to detect the mRNA expression of miR-10a-5p in T10 spinal cord of rats with SCI on the 21st day after operation. (B,C). The activation of microglia in T10 with SCI was detected by immunohistochemistry and WB on the 21st day after operation. (D). The BBB movement scale was used to monitor the neurological function of rats. (E).Pain-related behaviors, mechanical hypersensitivity reactions were determined by the withdrawal threshold. F. Temperature hypersensitivity was analyzed by withdrawal threshold. (G–I). The expressions of COX2, iNOS, IL-6, IL-1 β, IL-18, IFN-α, MDA and SOD were detected by WB and ELISA on the 21st day after operation. NS P > 0.05,**P < 0.01, ***P < 0.001.N = 5.
