Fig. 2

The effect of alcohol exposure on ROS accumulation at (A) 30 min, (B) 1 h, (C) 2 h, (D) 24 h, (E) 48 h and (F) 72 h. Cells were seeded in 96-well plates and treated with 200 mM, 300 mM, and 350 mM ethanol. Data is presented as percentage from the control. Results are expressed as mean of replicates ± SEM (n = 3–6). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.