Fig. 8

3 h pre-treatment of nanoformulated curcumin on ethanol induced ROS production at (A) 24 h, (B) 48 h and (C) 72 h. Cells were seeded in 96-well plates and treated with 350 mM ethanol after 3 h pre-treatment of formulations. ROS production was determined by the DCFDA assay. Data is presented as percentage from the control. Results presented as mean + SEM (n = 3) *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.