Fig. 4 | Scientific Reports

Fig. 4

From: Ethoxychelerythrine as a potential therapeutic strategy targets PI3K/AKT/mTOR induced mitochondrial apoptosis in the treatment of colorectal cancer

Fig. 4

Effect of Eth on the mitochondrial apoptosis of SW480 cells. (A–D) Flow cytometry analysis of MMP (A) and ROS (C) in Eth-treated SW480 cells and the quantification results (B,D), respectively. (E) Western blot analysis of PARP, Cyt C, and Cleaved caspase3 in Eth-treated SW480 cells. The relative protein densities of (F) PARP, (G) Cyt C, (H) Cleaved caspase3. (I) Effect of 32 μM Eth on the cell viability in the presence or absence of 10 μM Z-VAD-FMK was determined by MTT. Data shown are means ± SD (n = 6). *P < 0.05, **P < 0.01 compared to the CON. ##P < 0.01 compared to the 32 μM + 10 μM Z-VAD-FMK. Original blots of figure are presented in Supplementary Information.

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