Fig. 4

hsa_circ_0074763 overexpression promoted the activation, proliferation, inflammation of HSCs and inhibited their apoptosis. (A-D) The mRNA expressions of α-SMA and Col1A1 were detected by qRT PCR assay after LV-hsa_circ_0074763/si-hsa_circ_0074763 transfection. (E-J) The protein expressions of α-SMA and Col1A1 were detected by Western blot assay after LV-hsa_circ_0074763/si-hsa_circ_0074763 transfection. (K-L) Proliferation of LX-2 cells was detected by CCK8 assays after LV-hsa_circ_0074763/si-hsa_circ_0074763 transfection. (M-P) The protein expressions of ALT and AST by ELISA. (Q-T) The mRNA expressions of TNF-α and IL-6 were detected by qRT PCR assay after LV-hsa_circ_0074763/si-hsa_circ_0074763 transfection. (U) After transfections above, apoptosis was detected by Annexin V-APC-FITC double staining combined with flow cytometry was performed in each group. *P < 0.05; **P < 0.01; ***P < 0.001 n = 3.