Fig. 4 | Scientific Reports

Fig. 4

From: Endocardial-to-mesenchymal transition underlies cardiac outflow tract septation and bicuspid aortic valve formation in the Syrian hamster model

Fig. 4

Frontal (a–c) and transverse (d–l) sections of the middle OFT of ED11 hamster embryos. (a) Frontal semithin section of the fusion area stained with Toluidine Blue-Basic Fuchsin. Dashed lines mark the plane of sections shown in (d–f). (b,c,g–l) Histological sections immunostained with CD34 or VE-Cadherin (green) and α-actin (red). White arrowheads point to double stained cells, yellow arrowheads to intracellular VE-Cadherin, and white arrows to forming lamellipodia and filopodia. Nuclei are stained with DAPI (blue). (d–f) Overviews of the OFTs stained with hematoxylin-eosin showing the fusion process transversally. Black rectangles in d–f mark magnified areas shown in g,j; h,k and i,l, respectively. Scale bar for insets: 100 μm. Scale bar for magnifications: 25 μm.

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