Fig. 5 | Scientific Reports

Fig. 5

From: Endocardial-to-mesenchymal transition underlies cardiac outflow tract septation and bicuspid aortic valve formation in the Syrian hamster model

Fig. 5

Transverse (a–c, g–l) and frontal (d–f) sections of the middle region of the OFT of ED11 (a–f) and ED12 (g–l) hamster embryos. Normal (TAV) and BAV embryos are shown in (g-i) and (a–f), (j–l), respectively. Overviews in (a,d,g,j) are stained with hematoxylin-eosin. Sections immunostained with CD34 or VE-Cadherin (green) and α-actin (red) are shown in (b,c,e,f,h,i,k,l. Nuclei were stained with DAPI (blue). Black rectangles in a and d indicate magnified areas shown in b,c and e,f respectively. Yellow rectangles in h and k indicate magnified areas shown in i and l, respectively. White arrowheads point to double stained cells, yellow arrowheads to intracellular VE-Cadherin and white arrows to forming lamellipodia and filopodia. Asterisks in a and j indicate fused cushions in a BAV embryo. Scale bar for insets: 100 μm. Scale bar for magnifications: 50 μm.

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