Fig. 2

USV rates are low during interactions between female mice and muted males, independent of female estrous state. (A) Top panel shows experimental design and timeline for Experiment 1, and bottom panel shows experimental design and timeline for Experiments 2–3. (B) Total USVs are shown for courtship trials between muted males and group-housed B6 females (left), single-housed B6 females (middle), and single-housed outbred wild-derived females (right) according to female estrous state. M metestrus, D diestrus, P proestrus, E estrus. Panel (A) created in BioRender. Malone, C. (2024) https://BioRender.com/r97o719.