Fig. 4
From: BRD4 as the key lactylation related gene in heart failure identified through bioinformatics analysis
Construction of in vitro HF cell model and analysis of BRD4. (A) Cell viability assay using CCK8 after treating AC16 cells with different concentrations of DOX (0.5 µmol/L, 1 µmol/L, 1.5 µmol/L, and 2 µmol/L). Results indicate a significant decrease in AC16 cell viability with increasing DOX concentrations, with viability at approximately 75% at 1 µmol/L DOX and significantly decreasing to around 50% at 1.5 µmol/L DOX. (B) mRNA expression levels of BNP and ANP in the heart failure cell model treated with 1 µmol/L DOX, showing significant elevation compared to the control group, confirming successful model construction. (C) Western blot analysis of BRD4 protein expression in the heart failure cell model, demonstrating an increase in BRD4 protein levels with higher DOX concentrations. (D) Quantitative PCR analysis of BRD4 mRNA expression in the heart failure cell model, indicating elevated BRD4 mRNA levels compared to the control group.
