Fig. 1
From: Knockdown of LncRNA MEG3 promotes damage of vascular endothelial cells induced by vibration
Vibration-induced changes in viability, subcellular structure and apoptosis in HUVECs. (A) Effect of vibration at 125 Hz for 1–4 h on HUVEC viability. N = 3 experimental replicates per group. (B) Effect of vibration at different frequencies for 2.5 h (left) and 3 h (right) on one-day group HUVEC viability. N = 3 experimental replicates per group. (C) Effect of vibration at different frequencies for 2.5 h (left), 3 h (middle) and 4 h (right) on two-day group HUVEC viability. N = 3 experimental replicates per group. (D) Intracellular structural hanges were observed at different magnifications of 1 μm-10 kx, 2 μm-5 kx, 2 μm-4 kx, and 2 μm-5 kx. Nucleus pyknosis, chromatin margination, swollen endoplasmic reticulum and mitochondria, cell budding and vacuolization are indicated with red arrows. (E–F) Effect of vibration at 125 Hz (E) and 20 Hz (F) for 2.5 h on one-day group HUVEC apoptosis. N = 3 experimental replicates per group. (G–H) Effect of vibration at 125 Hz (G) and 20 Hz (H) for 2.5 h on two-day group HUVEC apoptosis. N = 3 experimental replicates per group. (I) Flow cytometry was used to measure % of apoptotic cells. N = 3 experimental replicates per group. *P < 0.05, **P < 0.01, compared with the control group.
