Fig. 2
From: A Stable and Dependable Visual Technique for On-Site Nipah Virus Nucleic Acids Detection
The principle of double antigen sandwich colloidal gold immunochromatography. (A) Colloidal gold-anti-FAM antibody (mouse anti-FAM) was placed on the binding pad. The test (T) line of anti-Digoxin antibody was fixed as the detection line. A fixed sheep anti-mouse polyclonal antibody served as (control) C line, which can combine mouse antibody; (B) In the presence of the target sequence, the amplified product, marked with Digoxin and FAM, can be generated using specific primers; (C) In the presence of the target product, a “colloidal gold - anti-FAM antibody - target product - anti-Digoxin antibody structure” was formed at the T-line to make the T-line color. At the position of line C, “Colloidal gold - anti-FAM antibody - sheep anti-mouse polyclonal antibody” was formed to make line C color; (D) Negative and positive results.
