Fig. 5
From: A Stable and Dependable Visual Technique for On-Site Nipah Virus Nucleic Acids Detection
Coincidence rate of simulated clinical sample assay. A, B: The results of simulated clinical sample detection targeting NiV P gene with RT-PCR and POC-NAD; C. Coincidence rate of conventional RT-PCR and POC-NAD with Kappa analysis. 1–16: we use the mixture of clinical sample RNA in 2.5µL and ddH2O in 2.5µL; 17–21: we use the mixture of clinical sample RNA in 2.5µL and the P and G gene RNA fragment in 1.25µL (6370 copies/µL); The total volume of the sample is 5µL; N: Negative control, template is H2O. P: positive control, template is P gene RNA fragment.
