Fig. 1
From: MiR-365-3p inhibits lung cancer proliferation and migration via CPT1A-mediated fatty acid oxidation
miR-365-3p inhibits CPT1A expression by targeting the 3’-UTR of CPT1A mRNA. (a)Western blot analysis of CPT1A protein expression in 293T cells transfected with negative control (NC) or mimics of candidate miRNAs as indicated. β-actin was used as loading control.(b)Western blot analysis of CPT1A protein expression in A549 and H1299 cells transfected with NC or miR-365-3p mimics. β-actin was used as loading control. Histograms show relative miR-365-3p expression. (c)qRT-PCR detection of CPT1A mRNA expression in A549 and H1299 cells transfected with NC or miR-365-3p mimics. (d) Luciferase activity assay was performed in cells co-transfected with CPT1A wild-type 3’-UTR (WT) or mutant 3’-UTR (MT) and NC or miR-365-3p mimics in A549 and H1299 cells. *, P < 0.05; **, P < 0.01.
