Fig. 2
From: MiR-365-3p inhibits lung cancer proliferation and migration via CPT1A-mediated fatty acid oxidation
miR-365-3p suppresses FAO through CPT1A in lung cancer cells.(a) Immunofluorescence staining to detect the level of lipid droplets (green) in A549 cells stably infected with lentivirus carrying CPT1A shRNA (shCPT1A) or control shRNA (shCtrl) and transfected with miR-365-3p mimics or NC. Scale bar, 10 μm. Histograms show relative lipid droplets level.(b) Oil Red O staining assay of A549 cells transfected as in (a). Scale bar, 10 μm. Histograms show relative lipid droplets level. (c) FAO assay of A549 cells transfected as in (a). Cells treated with FCCP or Etomoxir were used as positive and negative controls, respectively. Histograms show relative FAO rate. (d) ATP production assay of A549 cells transfected as in (a). The representative Western blot shows the expression of CPT1A. qRT-PCR analysis indicates the expression of miR-365-3p. *, P < 0.05; **, P < 0.01.
