Fig. 1

A mouse overload model combining treadmill exercise with weight attachment. (a) The protocol for the mouse overload model. The mice were euthanized 1, 4, or 24 h after single running (S1, S4, and S24), 24 h after the final running of 4 days (R24), or after 1 day without running (control). (b) Safranin O staining of the intercondylar region for the assessment of the synovium (left) and the medial compartment for the assessment of the cartilage in the control and R24 mice. Scale bars, 250 μm.